OmniKine^TM Colorimetric ELISA Kits
The OmniKine™ Colorimetric ELISA Kit contains the components necessary for quantitative determination of natural or recombinant cytokine concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a “Sandwich” Enzyme-Linked ImmunoSorbent Assay (ELISA) where the target protein (antigen) is bound in a “sandwich” format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are monoclonal, specific for a particular epitope on the protein of interest while the user-added detection antibodies are polyclonal for binding to a variety of epitopes on the bound target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and “sandwiching” of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the colorimetric substrate is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of proper stop solution where the color changes again to one that can be easily read by a spectrophotometer. The absorbance of each well is then read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
>> Click to view full list of OmniKine™ ELISA Kits

SensiKine^TM High Sensitivity Colorimetric ELISA Kits
The SensiKine™ High Sensitivity Colorimetric ELISA Kit contains the components necessary for highly sensitive detection and quantitative determination of natural or recombinant cytokines at low concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a “Sandwich” Enzyme-Linked ImmunoSorbent Assay (ELISA) where the target protein (antigen) is bound in a “sandwich” format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are monoclonal, specific for a particular epitope on the cytokine while the user-added detection antibodies are polyclonal for binding to a variety of epitopes on the bound target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and “sandwiching” of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the colorimetric substrate is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of stop solution where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
>> Click to view full list of SensiKine™ HS-ELISA Kits