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p21 Cip1 (Phospho-Thr145) Antibody
|H, M, R||WB IHC ELISA||17 kDa||Rabbit|
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
|Product Name||p21 Cip1 (Phospho-Thr145) Antibody|
|Synonyms||CAP20; CDK-interacting protein 1; CDKN1; CDKN1A; CDN1A; CIP1; Cyclin-dependent kinase inhibitor 1; MDA-6; MDA6; Melanoma differentiation associated protein; Melanoma differentiation associated protein 6; p21; PIC1; SDI1; WAF1|
|Immunogen||The antiserum was produced against synthesized peptide derived from human p21 Cip1 around the phosphorylation site of Thr145.|
|Modification sites||H:T145 M:T140 R:T145|
|Application dilutions||WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000|
|Specificity||p21 Cip1 (Phospho-Thr145) Antibody detects endogenous levels of p21 Cip1 only when phosphorylated at threonine145.|
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.|
|Physical Form||Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Storage/Stability||Store at -20℃/1 year|
|NCBI Gene Symbol||CDKN1A|
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p21 Cip1 (Phospho-Thr145) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa cells treated with EGF, using p21 Cip1 (Phospho-Thr145) Antibody. The lane on the left is treated with the synthesized peptide.
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