p21 Cip1 (Phospho-Thr145) Antibody

Catalog #	Size	Price
A7175		$150

Reactivity	Applications	MW.	Source
H, M, R	WB IHC ELISA	17 kDa	Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name	p21 Cip1 (Phospho-Thr145) Antibody
Synonyms	CAP20; CDK-interacting protein 1; CDKN1; CDKN1A; CDN1A; CIP1; Cyclin-dependent kinase inhibitor 1; MDA-6; MDA6; Melanoma differentiation associated protein; Melanoma differentiation associated protein 6; p21; PIC1; SDI1; WAF1
Immunogen	The antiserum was produced against synthesized peptide derived from human p21 Cip1 around the phosphorylation site of Thr145.
Immunogen Region	C-terminal
Clonality	Polyclonal
Modification sites	H:T145 M:T140 R:T145
Application dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity	p21 Cip1 (Phospho-Thr145) Antibody detects endogenous levels of p21 Cip1 only when phosphorylated at threonine145.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year

NCBI Gene Symbol

CDKN1A

Database Links

Gene ID:	1026	Swiss-Prot #:	P38936
OMIM #:	116899	Unigene #:	Hs.370771

Application images

p21 Cip1 (Phospho-Thr145) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p21 Cip1 (Phospho-Thr145) Antibody. The picture on the right is treated with the synthesized peptide.

p21 Cip1 (Phospho-Thr145) Antibody for Western Blot

Western blot analysis of extracts from HeLa cells treated with EGF, using p21 Cip1 (Phospho-Thr145) Antibody. The lane on the left is treated with the synthesized peptide.