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Home Antibodies Phospho-specific ACK1 (Phospho-Tyr284) Antibody

ACK1 (Phospho-Tyr284) Antibody

Catalog #SizePrice
A0604$150
　
ReactivityApplicationsMW.Source
H, MWB IHC IF ELISA114 kDaRabbit
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Product NameACK1 (Phospho-Tyr284) Antibody
SynonymsACK1; Activated p21cdc42Hs kinase; Non-receptor protein tyrosine kinase Ack; TNK2; kinase ACK1
ImmunogenThe antiserum was produced against synthesized peptide derived from human ACK1 around the phosphorylation site of Tyr284.
Immunogen RegionInternal
ClonalityPolyclonal
Modification sitesH:Y284 M:Y284
Application dilutionsWB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
SpecificityACK1 (Phospho-Tyr284) Antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine284.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical FormRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration1 mg/ml
Storage/StabilityStore at -20℃/1 year
NCBI Gene SymbolTNK2
Database Links
Gene ID:10188Swiss-Prot #:Q07912
OMIM #:606994Unigene #:Hs.518513
Application images
ACK1 (Phospho-Tyr284) Antibody for Immunofluorescence

Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is treated with the synthesized peptide.

ACK1 (Phospho-Tyr284) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is treated with the synthesized peptide.

ACK1 (Phospho-Tyr284) Antibody for Western Blot

Western blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30', using ACK1 (Phospho-Tyr284) Antibody. The lane on the right is treated with the synthesized peptide.

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