53BP1 (Phospho-Ser25) Antibody

Catalog #	Size	Price
A0886		$150

Reactivity	Applications	MW.	Source
H, M, R	IHC IF ELISA	213 kDa	Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name	53BP1 (Phospho-Ser25) Antibody
Synonyms	p53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1; Tumor suppressor p53-binding protein 1
Immunogen	The antiserum was produced against synthesized peptide derived from human 53BP1 around the phosphorylation site of Ser25.
Immunogen Region	N-terminal
Clonality	Polyclonal
Modification sites	H:S25 M:S25 R:S30
Application dilutions	IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
Specificity	53BP1 (Phospho-Ser25) Antibody detects endogenous levels of 53BP1 only when phosphorylated at serine25.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year

NCBI Gene Symbol

TP53BP1

Database Links

Gene ID:	7158	Swiss-Prot #:	Q12888
OMIM #:	605230	Unigene #:	Hs.440968

Application images

53BP1 (Phospho-Ser25) Antibody for Immunofluorescence

Immunofluorescence analysis of NIH-3T3 cells, using 53BP1 (Phospho-Ser25) Antibody. The picture on the right is treated with the synthesized peptide.

53BP1 (Phospho-Ser25) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 53BP1 (Phospho-Ser25) Antibody. The picture on the right is treated with the synthesized peptide.