p53 (Acetyl-Lys386) Antibody

Catalog #	Size	Price
D0017		$150

Reactivity	Applications	MW.	Source
H	IHC IF ELISA	43 kDa	Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name	p53 (Acetyl-Lys386) Antibody
Synonyms	Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53
Immunogen	The antiserum was produced against synthesized peptide derived from human p53 around the acetylated site of Lys386.
Immunogen Region	C-terminal
Clonality	Polyclonal
Modification sites	H:K386
Application dilutions	IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity	p53 (Acetyl-Lys386) Antibody detects endogenous levels of total p53 protein only when acetylated at lysine386.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
Physical Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year

NCBI Gene Symbol

TP53

Database Links

Gene ID:	7157	Swiss-Prot #:	P04637
OMIM #:	133239 / 151623 / 161550 / 191170 / 202300 / 211980 / 260500 / 275355	Unigene #:	Hs.654481

Application images

p53 (Acetyl-Lys386) Antibody for Immunofluorescence

Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys386) Antibody. The picture on the right is treated with the synthesized peptide.

p53 (Acetyl-Lys386) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 (Acetyl-Lys386) Antibody. The picture on the right is treated with the synthesized peptide.