NF-kappaB p65 (Acetyl-Lys310) Antibody

Catalog #	Size	Price
D0018		$150

Reactivity	Applications	MW.	Source
H, M	WB IHC IF ELISA	60 kDa	Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name	NF-kappaB p65 (Acetyl-Lys310) Antibody
Synonyms	NFKB3; nuclear factor NF-kappa-B p65 subunit; p65; RELA; TF65; transcription factor p65
Immunogen	The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the acetylated site of Lys310.
Immunogen Region	Internal
Clonality	Polyclonal
Modification sites	H:K310 M:K310
Application dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity	NF-kappaB p65 (Acetyl-Lys310) Antibody detects endogenous levels of total NF-kappaB p65 protein only when acetylated at lysine310.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
Physical Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year

NCBI Gene Symbol

RELA

Database Links

Gene ID:	5970	Swiss-Prot #:	Q04206
OMIM #:	164014	Unigene #:	Hs.502875

Application images

NF-kappaB p65 (Acetyl-Lys310) Antibody for Immunofluorescence

Immunofluorescence analysis of HeLa cells, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is treated with the synthesized peptide.

NF-kappaB p65 (Acetyl-Lys310) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is treated with the synthesized peptide.

NF-kappaB p65 (Acetyl-Lys310) Antibody for Western Blot

Western blot analysis of extracts from HeLa cells, treated with TSA 40nM 24h, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The lane on the right is treated with the synthesized peptide.