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Histone H3 (Acetyl-Lys18) Antibody
|H, M, R||WB IHC IF ELISA||15 kDa||Rabbit|
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
|Product Name||Histone H3 (Acetyl-Lys18) Antibody|
|Synonyms||H3/a; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31; HIST1H3A; histone H3.1|
|Immunogen||The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys18.|
|Modification sites||H:K18 M:K18 R:K18|
|Application dilutions||WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000|
|Specificity||Histone H3 (Acetyl-Lys18) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine18.|
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.|
|Physical Form||Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Storage/Stability||Store at -20℃/1 year|
|NCBI Gene Symbol||HIST1H3A / HIST1H3B / HIST1H3C / HIST1H3D / HIST1H3E / HIST1H3F / HIST1H3G / HIST1H3H / HIST1H3I / HIST1H3J / HIST2H3A / HIST2H3C / HIST2H3D / H3F3A / H3F3B|
Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys18) Antibody. The picture on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 (Acetyl-Lys18) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys18) Antibody. The lane on the right is treated with the synthesized peptide.
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