Histone H2B (Acetyl-Lys15) Antibody

Catalog #	Size	Price
D0005		$150

Reactivity	Applications	MW.	Source
H, M	WB IHC IF ELISA	13 kDa	Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name	Histone H2B (Acetyl-Lys15) Antibody
Synonyms	Histone cluster 1; H2bb,H2B.1; H2B/f; H2BFF; H2B histone family, member F,histone 1; H2bb
Immunogen	The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys15.
Immunogen Region	N-terminal
Clonality	Polyclonal
Modification sites	H:K15 M:K15
Application dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
Specificity	Histone H2B (Acetyl-Lys15) Antibody detects endogenous levels of total Histone H2B protein only when acetylated at lysine15.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
Physical Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year

NCBI Gene Symbol

H2BFS

Database Links

Gene ID:	54145	Swiss-Prot #:	P57053
OMIM #:		Unigene #:	Hs.473961

Application images

Histone H2B (Acetyl-Lys15) Antibody for Immunofluorescence

Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is treated with the synthesized peptide.

Histone H2B (Acetyl-Lys15) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is treated with the synthesized peptide.

Histone H2B (Acetyl-Lys15) Antibody for Western Blot

Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys15) Antibody. The lane on the right is treated with the synthesized peptide.